Pyrroline-5-caroxylate reductase, M. tuberculosis Recombinant | P1456

P5CR catalyzes the NADH dependent reaction of converting pyrroline-5-caboxylate to proline. It is important for regulating amino acid metabolism, intracellular redox potential, and apoptosis. Increased P5CR expression and activity have been observed in carcinoma and pulmonary tumors.

P1456 | Pyrroline-5-caroxylate reductase, M. tuberculosis Recombinant DataSheet

Biomolecule/Target :

Synonyms: P5CR, PCA reductase, P5C reductase, proC

Alternates names: P5CR, PCA reductase, P5C reductase, proC

Taglines: Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline

NCBI Gene ID #: 887256

NCBI Gene Symbol: proC

Gene Source: Mycobacterium tuberculosis

Accession #: P9WHU7

Recombinant: False

Source: E. coli

Purity by SDS-PAGE: ≥90%

Assay: SDS-PAGE

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method):

Biological activity: This enzyme has a specific activity of ≥ 7 mU/mg based on its conversion of proline to pyrroline-5-caboxylate.

Results:

Binding Capacity:

Unit Definition: One unit is the amount of enzyme that will convert 1.0 umole of proline to pyrroline-5-caborxylate per minute at pH 9.5 and 37 ÌŠC.

Molecular Weight: 49.4 kDa with N-terminal TRX tag

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute the lyophilized protein with 30% Glycerol and 5 mM BME at the concentration of 0.5 mg/ml. Incubate the reconstituted protein at 25 °C for 15 minutes.

Amino acid sequence: 1 – 295

Handling: Centrifuge the vial prior to opening.

Usage: