Rat 5HTR2C (5-Hydroxytryptamine Receptor 2C) ELISA Kit | ELK6222

Rat 5HTR2C (5-Hydroxytryptamine Receptor 2C) ELISA Kit | ELK6222 | Gentaur UK, US & Europe Distribution

Alternative Names: 5-HT2C; HTR1C; Serotonin Receptor 2C; 5-hydroxytryptamine receptor 1C

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxytryptamine Receptor 2C (5HTR2C) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptamine Receptor 2C (5HTR2C) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Hydroxytryptamine Receptor 2C (5HTR2C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine Receptor 2C (5HTR2C) in the samples is then determined by comparing the OD of the samples to the standard curve.