Rat 6ketoPGF1α (6-keto-PGF1 alpha) ELISA Kit | ELK8363

Rat 6ketoPGF1α (6-keto-PGF1 alpha) ELISA Kit | ELK8363 | Gentaur UK, US & Europe Distribution

Alternative Names: 6-keto-PGF1 alpha; 6ketoPGF1α

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 1.4 pg/ml

Standard: 500 pg/ml

Detection Range: 7.8 pg/ml - 500 pg/ml

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Neuro science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 6-keto-PGF1 alpha (6ketoPGF1α) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 6-keto-PGF1 alpha (6ketoPGF1α) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 6-keto-PGF1 alpha (6ketoPGF1α) in the samples is then determined by comparing the OD of the samples to the standard curve.