Red Fluorescent Protein - R-PE (R-Phycoerythrin) | 6005

R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE consists of a, b and g subunits and is present as (ab) 6g. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins having orange fluorescence. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates.

6005 | Red Fluorescent Protein - R-PE R-Phycoerythrin DataSheet

Biomolecule/Target: R-PE

Synonyms: RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin

Alternates names: soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL

Taglines: Fluorescent protein which can be cross linked or conjugated to other molecules to make fluorescent probes.

NCBI Gene ID #: 355

NCBI Gene Symbol: FASL

Gene Source: Human

Accession #: P25445

Recombinant: No

Source: CHO cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is 10.0 ng/ml, corresponding to a specific activity of 1 x 105 units/mg.

Biological activity: Determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is 10.0 ng/ml, corresponding to a specific activity of 1 x 105 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.9 kDa

Concentration: N/A

Appearance: Suspension

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.5x PBS, pH 7.5.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL