Resazurin sodium | B3296

Resazurin is a blue fluorogenic dye used as a redox indicator in cell viability and proliferation assays for bacteria, yeast or mammalian cells. The blue form of the dye is irreversibly reduced by enzymes in viable cells to generate a highly red-fluorescent product, resorufin, which displays absorption/emission maxima ~575/585 nm and can be detected by flow cytometry, fluorescence microscopy, and high-throughput screening methods. Resazurin is minimally toxic to living cells, making it suitable for use in long-term cell culture.

Resazurin is a blue fluorogenic dye used as a redox indicator in cell viability and proliferation assays for bacteria, yeast or mammalian cells. The blue form of the dye is irreversibly reduced by enzymes in viable cells to generate a highly red-fluorescent product, resorufin, which displays absorption/emission maxima ~575/585 nm and can be detected by flow cytometry, fluorescence microscopy, and high-throughput screening methods. Resazurin is minimally toxic to living cells, making it suitable for use in long-term cell culture.

B3296 | Resazurin sodium DataSheet

Alternate Name/Synonyms: 7-hydroxy-3H-phenoxazin-3-one-10-oxide, monosodium salt

Appearance: Dark green to black powder

Formulation:

CAS Number: 62758-13-8

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₂H₆NO₄ • Na

Molecular Weight: 251.17

Cell-Permeable?: True

Purity: ~85% by HPLC

Solubilities: ~0.5 mg/ml Ethanol ~0.5 mg/ml DMSO

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A blue fluorogenic dye

MDL Number: MFCD00005036

PubChem CID: 112939

SMILES: C1=CC2=C(C=C1[O-])OC3=CC(=O)C=CC3=[N+]2[O-].[Na+]

InChi: InChI=1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1

InChi Key: IVGPGQSSDLDOLH-UHFFFAOYSA-M