RNF121 Antibody | 8285

RNF121 Antibody | 8285 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: RNF121 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RNF121.
The immunogen is located within amino acids 260 - 310 of RNF121.

Research Area: Stem Cell

Tested Application: E, WB, ICC

Application: RNF121 antibody can be used for detection of RNF121 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 10 μg/ml.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Specificiy: RNF121 antibody is human, mouse and rat reactive. Multiple isoforms of RNF121 are known to exist.

Positive Control 1: Cat. No. 1219 - MCF7 Cell Lysate

Positive Control 2: Cat. No. 17-012 - MCF7 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 24, 27, 36 kDa
Observed: 28 kDa

Validation: N/A

Isoform: N/A

Purification: RNF121 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: RNF121 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: RNF121 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Ring finger protein 121

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: RNF121 is an evolutionarily conserved E3 ligase ring finger protein that is expressed in the endoplasmic reticulum (1) . It acts downstream of the unfolded protein response regulator protein kinase-like endoplasmic reticulum kinase (PERK) . Overexpression of RNF121 during the early stages of gonad development led to aberrations in germline development and gonad migration, suggesting that it plays a role in endoplasmic reticulum-associated degradation machinery (1) .