Description
RNF121 Antibody | 8285 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: RNF121 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RNF121.
The immunogen is located within amino acids 260 - 310 of RNF121.
Research Area: Stem Cell
Tested Application: E, WB, ICC
Application: RNF121 antibody can be used for detection of RNF121 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 10 μg/ml.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificiy: RNF121 antibody is human, mouse and rat reactive. Multiple isoforms of RNF121 are known to exist.
Positive Control 1: Cat. No. 1219 - MCF7 Cell Lysate
Positive Control 2: Cat. No. 17-012 - MCF7 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 24, 27, 36 kDa
Observed: 28 kDa
Validation: N/A
Isoform: N/A
Purification: RNF121 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: RNF121 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: RNF121 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Ring finger protein 121
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: RNF121 is an evolutionarily conserved E3 ligase ring finger protein that is expressed in the endoplasmic reticulum (1) . It acts downstream of the unfolded protein response regulator protein kinase-like endoplasmic reticulum kinase (PERK) . Overexpression of RNF121 during the early stages of gonad development led to aberrations in germline development and gonad migration, suggesting that it plays a role in endoplasmic reticulum-associated degradation machinery (1) .