sCD22, Human Recombinant | 7123

CD22 is a B-lineage restricted 135 kDa glycoprotein whose cell surface expression is limited to resting and activated B lymphocytes. The physiological role of CD22 is still unknown. Targeted disruption of CD22 in mice results in a reduced level of surface IgM on peripheral B cells suggesting a role for CD22 in limiting antigen receptor signaling. CD22 is a member of the Ig gene superfamily that uniquely binds a sialic acid-dependent ligand. Recombinant human CD22 is a soluble 75.0 kDa protein which corresponds to the extracellular domain of CD22.

7123 | sCD22 Human Recombinant DataSheet

Biomolecule/Target: sCD22

Synonyms: Leu-14, B-Lymphocyte cell adhesion molecule, BL-CAM, Siglec-2

Alternates names: CXCL6

Taglines: Plays a potential role in limiting antigen receptor signaling

NCBI Gene ID #: 6372

NCBI Gene Symbol: CXCL3

Gene Source: Human

Accession #: P80162

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to chemoattract human neutrophils using a concentration range of 10.0-50.0 ng/ml.

Biological activity: Determined by its ability to chemoattract human neutrophils using a concentration range of 10.0-50.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 7.9 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM acetic acid.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: VLTELRCTCL RVTLRVNPKT IGKLQVFPAG PQCSKVEVVA SLKNGKQVCL DPEAPFLKKV IQKILDSGNK KN