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sCD27 Ligand, Human Recombinant | 7125
- SKU:
- 26-7125-GEN
- Availability:
- Usually shipped in 5 working days
Description
CD27 Ligand, a type II transmembrane protein, is a member of the TNF superfamily. It is expressed on activated T and B lymphocytes as well as NK cells. CD27L and its receptor (CD27) regulate the immune response by promoting T-cell expansion and differentiation, as well as NK enhancement. CD27 signaling can act as a co-stimulatory effector to sustain the survival of CD8+ T cells, primarily by inducing increased expression of the IL-2 gene. Full length human CD27L is a 193 amino acid protein, consisting of a 17 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 155 amino acid extracellular domain. Human soluble CD27L corresponds to the 155 amino acid extracellular domain of the full length CD27L protein. s recombinant human sCD27L contains the extracellular domain plus an N-terminal His-Tag.
7125 | sCD27 Ligand Human Recombinant DataSheet
Biomolecule/Target: sCD27
Synonyms: TNFSF7, CD70
Alternates names: Growth/Differentiation Factor-2, BMP-9
Taglines: Regulate the immune response by promoting T-cell expansion and differentiation
NCBI Gene ID #: 2658
NCBI Gene Symbol: GDF2
Gene Source: Human
Accession #: Q9UK05
Recombinant: Yes
Source: CHO cells
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.9 ng/ml.
Biological activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.9 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 24.1 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: SAGAGSHCQK TSLRVNFEDI GWDSWIIAPKE YEAYECKGGC FFPLADDVTPTK HAIVQTLVHL KFPTKVGKAC CVPTKLSPIS VLYKDDMGVP TLKYHYEGMS VAECGCR