SFTPD, human recombinant | 7328

SFTPD, as known as Surfactant pulmonary-associated protein D, is a member of the collectin family of C-type lectins that is synthesized in many tissues including respiratory epithelial cells in the lung, and contains one C-type lectin domain and one collagen-like domain. It is humoral molecules of the innate immune system, and is considered a functional candidate in chronic periodontitis. Besides it is involved in the development of acute and chronic inflammation of the lung. Several human lung diseases are characterized by decreased levels of bronchoalveolar SFTPD. Recombinant human SFTPD protein, fused to His-tag at N-terminus, was expressed in E.coli.

7328 | SFTPD human recombinant DataSheet

Biomolecule/Target: SFTPD

Synonyms: Pulmonary surfactant-associated protein D precursor, COLEC7, PSP-D, SFTP4, SP-D

Alternates names: Pulmonary surfactant-associated protein D precursor, COLEC7, PSP-D, SFTP4, SP-D

Taglines: Involved in the development of acute and chronic inflammation of the lung

NCBI Gene ID #: 60427

NCBI Gene Symbol: SCF

Gene Source: Human

Accession #: P21581

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 microgram of protein

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent stimulation of the proliferation of human MO7e cells is <10 ng/ml, corresponding to a specific activity of >1 x 10 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 18.9 kDa (175 aa, 224-375 aa + His Tag), confirmed by MALDI-TOF.

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, and 0.4 M Urea.

Reconstitution Instructions: Reconstitute with sterile 10 mM acetic acid to a concentration of 0.1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A