SGK2, Active | 7749

SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the "AGC" kinase subfamily, which includes protein kinases A, G, and C, and its catalytic domain is most similar to protein kinase B (PKB). SGK1 was originally identified as a glucocorticoid-sensitive gene and subsequently, the two homologous kinases SGK2 and SGK3 have been cloned, being products of distinct genes, which are differentially expressed and share 80% identity in amino acid sequence in their catalytic domains . SGK2, like the other two isoforms SGK1 and SGK3, is stimulated by insulin and insulin-like growth factor-1 (IGF-1), and has been shown to enhance Na(+)/K(+)-ATPase activity in a variety of cells. In addition, SGK2 mimics the function of SGK1 and SGK3 and participate in the regulation of renal epithelial Na(+) channel ENaC activity. SGK2 is activated by phosphorylation in response to signals that stimulate phosphatidylinositol 3-kinase by a huge number of extracellular signals. The phosphorylation of SGK2 is mediated by 3-phosphoinositide-dependent protein kinase 1 (PDK1) and other protein kinases that have yet to be identified. The substrate specificity of SGK isoforms superficially resembles that of PKB in that serine and threonine residues lying in Arg-Xaa-Arg-Xaa-Xaa-Ser/Thr sequences (where Xaa is a variable amino acid) are phosphorylated. However, although they may have some substrates in common, evidence is emerging that SGKs and PKB phosphorylate distinct proteins and have different functions in vivo. In particular, SGKs play an important role in activating certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Moreover, sustained high levels of SGK protein and activity may contribute to conditions such as hypertension and diabetic nephropathy.

7749 | SGK2 Active DataSheet

Biomolecule/Target: N/A

Synonyms: SGK, serum/glucocorticoid regulated kinase 2

Alternates names: SGK, serum/glucocorticoid regulated kinase 2

Taglines: A serine/threonine-protein kinase

NCBI Gene ID #: Ensembl: ENSMMUT00000015626

NCBI Gene Symbol: SAA

Gene Source: N/A

Accession #: P02738

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 82 nmol phosphate incorporated into Akt/SGK substrate peptide (RPRAATF) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 71.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in sterile ddHO to a concentration 100 µg/ml. This solution can then be diluted into other aqueous buffers.

Amino acid sequence: RSWFSFLGEA YDGARDMWRA YSDMKEANYK NSDKYFHARG NYDAAQRGPG GVWAAEVISD ARENIQKLLG RGAEDTLADQ AANEWGRSGK DPNHFRPAGL PEKY