sIL-4R, Human Recombinant | P1287

IL-4 can signal through type I and type II receptor complexes, which share a common chain (c). The type I receptor contains in addition to the chain an IL-4R subunit, whereas the type II receptor contains the IL-13R. The secreted extracellular domain of IL-4R, called sIL-4R, binds IL-4 and antagonizes its activity. It plays an important role in regulating the differentiation of naive CD4 T cells and class switching to IgG1 and IgE. Recombinant human sIL-4R is a 209 amino acid protein which corresponds to the entire extracellular domain of IL-4R.

P1287 | sIL-4R Human Recombinant DataSheet

Biomolecule/Target: sIL-4R

Synonyms: soluble IL-4 receptor alpha, CD124, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, sIL4Ralpha/prot, IL-4-binding protein, IL4-BP

Alternates names: IL-28A

Taglines: Plays an important role in regulating the differentiation of naive CD4 T cells and class switching to IgG1 and IgE.

NCBI Gene ID #: 282616

NCBI Gene Symbol: IFNL2

Gene Source: Human

Accession #: Q8IZJ0

Recombinant: Yes

Source: E Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to activate STAT phosphorylation in an ISRE Luciferase Reporter Assay using human colon carcinoma COLO205 cells. The expected ED50 is 0.3-0.5 ng/ml

Biological activity: Determined by its ability to activate STAT phosphorylation in an ISRE Luciferase Reporter Assay using human colon carcinoma COLO205 cells. The expected ED50 is 0.3-0.5 ng/ml

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.6 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Acetic Acid.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: PVARLHGALP DARGCHIAQF KSLSPQELQA FKRAKDALEE SLLLKDCRCH SRLFPRTWDL RQLQVRERPM ALEAELALTL KVLEATADTD PALVDVLDQP LHTLHHILSQ FRACIQPQPT AGPRTRGRLH HWLYRLQEAP KKESPGCLEA SVTFNLFRLL TRDLNCVASG DLCV