Sirtuin 2, human recombinant | 7632

Human sirtuins function as intracellular regulatory proteins. Sirtuin 2 is a member of the class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. The enzymatic activity of class III HDACs is nicotinamide adenine dinucleotide (0.1mM NAD+) dependent and insensitive to HDAC inhibitor trichostatin A.

7632 | Sirtuin 2 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Regulatory protein SIR2 homolog 2, SIR2-like protein 2

Alternates names: IBP-2

Taglines: A NAD-dependent deacetylase

NCBI Gene ID #: 3485

NCBI Gene Symbol: IGFBP2

Gene Source: Human

Accession #: P18065

Recombinant: Yes

Source: (BTI-Tn-5B1-4) Hi-5 Insect cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.

Biological activity: Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 31.5 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1 x PBS, pH 7.2.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: EVLFRCPPCT PERLAACGPP PVAPPAAVAA VAGGARMPCA ELVREPGCGC CSVCARLEGE ACQVYTPRCG QGLRCYPHPG SELPLQALVM GEGTCEKRRD AEYGASPEQV ADNGDDHSEG GLVENHVDST MNMLGGGGSA GRKPLKSGMK ELAVFREKVT EQHRQMGKGG KHHLGLEEPK KLRPPPARTP CQQELDQVLE RISTMRLPDE RGPLEHLYSL HIPNCDKHGL YNLKQCKMSL NGQRGECWCV NPNTGKLIQG APTIRGDPEC HLFYNEQQEA RGVHTQRMQ