SMN Tudor Domain (73-173 aa) GST-tagged), Human recombinant | 7676

Tudor domains are small protein structural motifs of ~50 amino acids related to the “Royal family” of methyl readers, which also includes chromo, MBT, PWWP, and Agenet-like domains. Tudor domains occur either alone, in tandem, or with other domains and are found in many proteins that are involved in RNA metabolism, germ cell development, transposon silencing, DNA damage response, histone modification and chromatin remodeling. The tudor domains recognize symmetric methylated arginine or methylated lysine residues. The Survival of Motor Neurons (SMN) protein participates in RNA splicing. The Tudor domain of SMN recognizes and binds methylated Sm proteins, which bind small nuclear RNA. SMN is encoded in humans by two separate genes, SMN1 and SMN2, which differ by one base in exon 7. In motor neuron cells, approximately 90% of the SMN2 transcripts are spliced to exclude exon 7. The SMN2 transcripts without exon 7 are less stable than SMN1 transcripts. Consequently, defects in human SMN1 result in the death of motor neuron cells and spinal muscular atrophy, which is the leading genetic cause of infantile death. This protein product contains the tudor domain region of SMN. The sequence of this region is identical in both the SMN1 and the SMN2 genes.

7676 | SMN Tudor Domain 1472-1613 aa GST-tagged Human recombinant DataSheet

Biomolecule/Target: SMN1 SMN2

Synonyms: SMN Tudor Domain (1472-1613 aa) (GST-tagged), Human recombinant

Alternates names: Insulin-like Growth Factor-Binding Protein 4, IBP-4, HT29-IGF-BP, colon cancer cell growth inhibitor

Taglines: Participates in RNA Splicing

NCBI Gene ID #: 3487

NCBI Gene Symbol: IGFBP4

Gene Source: Human

Accession #: P22692

Recombinant: Yes

Source: (BTI-Tn-5B1-4) Hi-5 Insect cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.

Biological activity: Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25.7 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1 x PBS, pH 7.2.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGelDCHQ LADSFRE