Sperm Antibodies ELISA kit | 55R-IB79154

Sperm Antibodies ELISA kit | 55R-IB79154 |

Activity Code: ACTIVE

Product Type: Research Kits

Product Subtype: Kits

Research Area: Neuroscience

Short Description: ELISA kit for the detection of Sperm Antibodies in the research laboratory

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: N/A

Residues: N/A

Tag/conjugate: N/A

Protein Type: N/A

Expression System: N/A

Grade & Purity: N/A

Methode of Purification: N/A

Source: N/A

Concentration: N/A

Form & Buffer: N/A

Storage: Store at 2-8 deg C

Shipping Information: *Blue Ice*

Applications: ELISA

Bioactivity: N/A

Usage Recommendations: Optimal conditions to be determined by end-user

Assay Information: The antispermatozoa antibody ELISA (Enzyme Linked ImmunoSorbent Assay) from IBLAmerica is a solidphase  sandwich enzymeimmunoassay for the quantitative determination of antispermatozoa antibodies in human serum.  The ELISAplate is coated with a mix of spermatozoa proteins which are recognized by antispermatozoa antibodies. The samples and standards are pipetted into the wells and then incubated. During this incubation antispermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. After washing the enzyme conjugate, consisting of antihuman globulin antibodies covalently coupled to horseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3, 3’, 5, 5’tetramethylbenzidine) yielding a colour reaction which is stopped with 0.25 M sulphuric acid (H2SO4) . The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength of 550 nm or higher is recommended.