sTRAIL Receptor-1/DR4, human recombinant | 7206

TRAIL Receptor-1/DR4 and TRAIL Receptor-2/DR5 belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain,” which can activate the cell’s apoptotic machinery. These receptors are activated by binding to either membrane anchored or soluble TRAIL/Apo2L. Recombinant human soluble TRAIL Receptor-1/DR4 is a 22.7 kDa protein (215 amino acid residues) consisting of the TNFR homologous, cysteine rich portion of the extracellular domain.

7206 | sTRAIL Receptor-1/DR4 human recombinant DataSheet

Biomolecule/Target: TNFRSF10A

Synonyms: soluble TRAIL Receptor-1, DR4, TNFRSF10A, Apo2

Alternates names: Monokine Induced by Interferon-, CXCL9

Taglines: Activates the cell’s apoptotic machinery

NCBI Gene ID #: 17329

NCBI Gene Symbol: CXCL9

Gene Source: Murine

Accession #: P18340

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to chemoattract human lymphocytes using a concentration range of 0.1-1.0 ng/ml.

Biological activity: Determined by its ability to chemoattract human lymphocytes using a concentration range of 0.1-1.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 12.2 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Acetic acid.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: TLVIRNARCS CISTSRGTIH YKSLKDLKQF APSPNCNKTE IIATLKNGDQ TCLDPDSANV KKLMKEWEKK INQKKKQKRG KKHQKNMKNR KPKTPQSRRR SRKTT