sTREM-1 (Human) ELISA Kit - E4935

E4935 | sTREM-1 (Human) ELISA Kit DataSheet

The triggering receptor expressed on myeloid cells-1 (TREM-1) is a type I transmembrane immune receptor that belongs to the TREM family of protein receptors. It is expressed on neutrophils and mature macrophages/monocytes. TREM-1 plays a significant role in the amplification of inflammation and activation of antigen-presenting cells (APC). A soluble form of TREM-1 (sTREM-1) is found in the plasma and is thought to arise from the proteolytic cleavage of membrane-associated TREM-1 by metalloproteinases (MMPs). Increased levels of sTREM-1 are observed during infection and inflammatory diseases such as inflammatory bowel disease, rheumatoid arthritis, renal IR. Therefore, sTREM-1 is a promising serological biomarker for these diseases. ’s sTREM-1 (Human) ELISA kit is based on the Sandwich principle to quantitatively measure the concentration of sTREM-1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of sTREM-1 in the sample or standard

Alternate Name : TREM1, CD354, TREM-1, CD354 Antigen

Tag Line : A Sandwich ELISA kit to quantitatively measure soluble TREM-1 in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma Serum Tissue lysate Other biological fluids

Species Reactivity : Human

Applications : This Sandwich ELISA kit is designed to quantitatively measure amount of sTREM-1 in human serum, plasma, tissue lysates, and other biological fluids

Features and Benefits : This Sandwich ELISA is highly sensitive and highly specific for the detection of sTREM-1 in human samples. There is no significant cross-reactivity or interference between sTREM-1 and analogues Detection range: 31.25 - 2000 pg/ml Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10% Recovery range: 85 - 105% for normal human serum and plasma samples Sensitivity: 18.75 pg/ml