TFF1, human recombinant | 4893

The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. Recombinant human TFF1 is a 8.2 kDa polypeptide containing 60 amino acid residues.

4893 | TFF1 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: pS2 protein, HP1.A, Breast Cancer Estrogen-Inducible protein (BCEI), PNR2

Alternates names: NPO, NP

Taglines: Involved in intestinal mucosal defense and repair

NCBI Gene ID #: 244218

NCBI Gene Symbol: Ctf2

Gene Source: Murine

Accession #: P83714

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is 0.5-0.8 g/ml.

Biological activity: Determined by the dose dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is 0.5-0.8 g/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.8 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 2.5 mM Tris, pH 10.2 and 0.5 mM DTT

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MAPISPSEPI GQAYSLALYM QKNTSALLQT YLQHQGSPFS DPGFSAPELQ LSTLPSAAVS FKTWHAMEDA ERLSRAQGAF LALTQHLQLV GDDQSYLNPG SPILLAQLGA ARLRAQGLLG NMAAIMTALG LPIPPEEDTL GFVPFGASAF ERKCRGYIVT REYGHWTDRA VRDLALLKAK YSA