TGF-3, Mouse Recombinant | 7588

The Transforming Growth Factors (TGFs) are multifunctional peptides that regulate growth and differentiation in a variety of cells. Recent data suggests that individual TGF- isoforms (TGF-1, -2 and –3) have overlapping, yet distinct biological actions and target cell specificities, both in developing and adult tissues. TGF-3 is a new isoform that is presumed to play an important role in wound repair and scarring. TGF-3 is also thought to be involved in osteoblast proliferation, chemotaxis, and collagen synthesis. Recombinant mouse TGF-3 is a non-glycosylated, disulfide-linked homodimer, containing two 112 amino acid chains, with a total molecular weight of 25.5 kDa.

7588 | TGF-3 Mouse Recombinant DataSheet

Biomolecule/Target: TGF-beta-1

Synonyms: Transforming growth factor beta-3

Alternates names: Platelet endothelial cell adhesion molecule, CD31 antigen, EndoCAM

Taglines: Plays an important role in wound repair/scarring, osteoblast proliferation, chemotaxis, and collagen synthesis

NCBI Gene ID #: 5175

NCBI Gene Symbol: PECAM1

Gene Source: Human

Accession #: P16284

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5 g/ml.

Biological activity: Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5 g/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 80-95 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium phosphate, pH 7.5

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: ENSFTINSVD MKSLPDWTVQ NGKNLTLQCF ADVSTTSHVK PQHQMLFYKD DVLFYNISSM KSTESYFIPE VRIYDSGTYK CTVIVNNKEK TTAEYQLLVE GVPSPRVTLD KKEAIQGGIV RVNCSVPEEK APIHFTIEKL ELNEKMVKLK REKNSRDQNF VILEFPVEEQ DRVLSFRCQA RIISGIHMQT SESTKSELVT VTESFSTPKF HISPTGMIME GAQLHIKCTI QVTHLAQEFP EIIIQKDKAI VAHNRHGNKA VYSVMAMVEH SGNYTCKVES SRISKVSSIV VNITELFSKP ELESSFTHLD QGERLNLSCS IPGAPPANFT IQKEDTIVSQ TQDFTKIASK SDSGTYICTA GIDKVVKKSN TVQIVVCEML SQPRISYDAQ FEVIKGQTIE VRCESISGTL PISYQLLKTS KVLENSTKNS NDPAVFKDNP TEDVEYQCVA DNCHSHAKML SEVLRVKVIA PVDEVQISIL SSKVVESGED IVLQCAVNEG SGPITYKFYR EKEGKPFYQM TSNATQAFWT KQKASKEQEG EYYCTAFNRA NHASSVPRSK ILTVRVILAP WK