Thrombin, Active, Bovine Plasma (High Activity) | 7592

Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.

7592 | Thrombin Active Bovine Plasma High Activity DataSheet

Biomolecule/Target: Thrombin

Synonyms: Activated Factor IIa

Alternates names: PSP, PSPN

Taglines: A coagulation protein and a serine protease.

NCBI Gene ID #: 5623

NCBI Gene Symbol: PSPN

Gene Source: Human

Accession #: O60542

Recombinant: No

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Human Persephin induces RET phosphorylation using a concentration range of 0.1-1.0 ng/ml. Human Persephin binds to mammalian GFR4 with the Kd of 100pM. Other members of the GDNF family (Artemin, GDNF and Neurturin) do not bind to mammalian GFR4.

Biological activity: Human Persephin induces RET phosphorylation using a concentration range of 0.1-1.0 ng/ml. Human Persephin binds to mammalian GFR4 with the Kd of 100pM. Other members of the GDNF family (Artemin, GDNF and Neurturin) do not bind to mammalian GFR4.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 10.4 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.3 x PBS, pH 7.2

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate, pH 7.5 to a concentration of 0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: RALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRH RWQRLPQLSA AACGCGG