Thymosin-4, human recombinant | 7216

Thymosin-4 is a small, actin-sequestering protein belonging to the thymosin- family that is found at high concentrations within the spleen, thymus, and peritoneal macrophages, where it is most notably responsible for the organization of cytoskeletal structure. In mammalian tissues, this protein acts as a modulator for the polymerization/depolymerization of actin through the formation of a 1:1 complex with the monomer G (globular)-actin, and inhibits actin’s polymerization to form F (filamentous) actin, which together with other proteins binds microfilaments to construct the cytoskeleton. Commonly found at significant quantities within the brain, lungs, liver, kidneys, testes, and heart, Thymosin-4 has also been shown to be synthesized by cells unrelated to the reticulo-endothelial system, such as myoblasts and fibroblasts, and expressed at irregular levels by several hemopoietic cell lines, malignant lymphoid cells and myeloma cells. In addition to regulating actin polymerization, research has also found Thymosin-4 to stimulate the secretion of hypothalamic luteinizing hormone-releasing hormone and luteinizing hormone, inhibit the migration of peritoneal macrophages, induce phenotypic changes in T cell lines during early host defense mechanisms, and inhibit the progression of hematopoietic pluripotent stem cells into the s-phase. Recombinant Human Thymosin-4 is a 5.2 kDa glycoprotein containing 45 amino acid residues.

7216 | Thymosin-4 human recombinant DataSheet

Biomolecule/Target: Thymosin-4

Synonyms: T-4, Hematopoietic system regulatory peptide, Seraspenide, TMSB4X, TB4X, TMSB4

Alternates names: Platelet Factor-4, CXCL4, Oncostatin A, Ironplact

Taglines: A small, actin-sequestering protein

NCBI Gene ID #: 56744

NCBI Gene Symbol: CXCL4

Gene Source: Mouse

Accession #: Q9Z126

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to chemoattract human neutrophils using a concentration range of 10.0-100.0 ng/ml.

Biological activity: Determined by its ability to chemoattract human neutrophils using a concentration range of 10.0-100.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 10.3 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES