TMB, Ultrasensitive | 1215

3,3'5,5'-Tetramethylbenzidine (TMB) has been shown to be a safe-sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum at 653 nm. Further reaction with HRP/H₂O₂ or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 3.9 x 10⁴ mo⁻¹1 cm⁻¹) and diimine (E450 nm = 5.9 x 10⁴ mol⁻¹ cm⁻¹) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMBUS, available from Inc., is a single component reagent stable at room temperature and not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP usually employed in immunologic assays.

TMB, Ultrasensitive has been shown to be a safe-sensitive substrate for the assay of horseradish peroxidase (HRP).

1215 | TMB, Ultrasensitive DataSheet

Alternate Name/Synonyms: 3,3',5,5'-Tetramethylbenzidine; TMBUS

Appearance: Liquid

Formulation: 2.08 mMol L⁻¹ and Hydrogen Peroxide, citric acid buffer at pH 3.3.

CAS Number: 54827-17-7

Structure Available?: No

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₆H₂₀N₂

Molecular Weight: 240.35

Cell-Permeable?: No

Purity: N/A

Solubilities: N/A

Handling: Protect from light

Country of Origin: USA

Tag Line: A peroxidase substrate

MDL Number: MFCD00007748

PubChem CID: 41206

SMILES: CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

InChi: InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChi Key: UAIUNKRWKOVEESUHFFFAOYSA-N