TNF Receptor-Associated Protein 1 (TRAP1), Human Recombinant | P1584

The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress. It includes cytoplasmic (Hsp90-alpha/beta), ER (Grp94) and mitochondrial (TRAP1) localized members. Hsp90 is structurally composed of an N-terminal ATP binding domain, a medial substrate-binding region, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones to stabilize a multitude of client proteins substrates. TRAP1 (aka Hsp75) was originally identified as a chaperone binding partner for retinoblastoma protein. TRAP1 was later found by immunofluorescence to be localized to the mitochondria. TRAP1 is significantly more active as an ATPase due in part to the lack of regulatory elements in the C-terminus, but retains the ability to dimerize. However, it does not form stable complexes with typical cochaperones of Hsp90 like p23 and HOP4. TRAP1 has been identified as a protective element for cell survival, and has been suggested as a target for anti-cancer therapeutics.

P1584 | TNF Receptor-Associated Protein 1 (TRAP1), Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: HSP75, Mitochondrial HSP90, MtHSP90

Alternates names: HSP75, Mitochondrial HSP90, MtHSP90

Taglines: Interacts with EXT2, EXT1 and Retinoblastoma protein

NCBI Gene ID #: 10131

NCBI Gene Symbol: TRAP1

Gene Source: Human

Accession #: Q12931

Recombinant: TRUE

Source: E. coli

Purity by SDS-PAGE: >95%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level: <50 EU/mg purified protein (LAL test)

Activity (Specifications/test method):

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: ~72kDa (N terminus His-Tag)

Concentration:

Appearance: Liquid

Physical form description: In 50 mM TRIS, pH 7.5, containing 150 mM sodium chloride, 1 mM DTT, and 1 mM EDTA.

Reconstitution Instructions:

Amino acid sequence: aa 60-704

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans