TRAIL, murine recombinant | 7205

TRAIL is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain”, which activates the cell’s apoptotic machinery. Recombinant murine TRAIL is a 174 amino acid polypeptide (20.0 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein.

7205 | TRAIL murine recombinant DataSheet

Biomolecule/Target: TRAIL

Synonyms: TNF-related apoptosis-inducing Ligand, TNFSF10, Apo2 Ligand, TL2

Alternates names: Stromal-Cell Derived Factor-1, CXCL12, PBSF

Taglines: Activates rapid apoptosis in tumor cells

NCBI Gene ID #: 24772

NCBI Gene Symbol: SDF-1

Gene Source: RAT

Accession #: Q9QZD1

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50.0-100.0 ng/ml.

Biological activity: Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50.0-100.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 7.9 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK