TurboNuclease, Recombinant | 9207

’s TurboNuclease is a recombinant form of Serratia macescens extracellular endonuclease (encoded by the same gene of Benzonase) produced in E. coli using a proprietary process. This nonspecific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptional high specific activity and is free of protease activity. It is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.

9207 | TurboNuclease, Recombinant DataSheet

Biomolecule/Target :

Synonyms: TurboNuclease

Alternates names: TurboNuclease

Taglines: A nonspecific endonuclease

NCBI Gene ID #: N/A

NCBI Gene Symbol: N/A

Gene Source: N/A

Accession #: N/A

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGE: N/A

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: >1.3x10⁶ units/mg. One unit of TurboNuclease converts 50 ug of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl₂.

Molecular Weight: 27.0 kDa

Concentration: 250 U/ul

Appearance: Liquid

Physical form description: 250 units/ul in a storage buffer of 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgClâ‚‚ and 50% glycerol

Reconstitution Instructions: N/A

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans