UbcH2, human recombinant (His-tag) | 4848

UbcH2 is the human homolog to the yeast DNA repair gene RAD6, which is induced by DNA damaging reagents. It has been shown to conjugate ubiquitin to histone H2A in an E3 dependent manner in vitro. UbcH2 consists of two functionally independent domains, an N-terminal core domain with ubiquitin conjugating activity, and a C-terminal domain that interacts with substrate proteins. This protein is useful for in vitro ubiquitinylation reactions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 M depending upon conditions.

4848 | UbcH2 human recombinant His-tag DataSheet

Biomolecule/Target: N/A

Synonyms: UbcH2, Ubiquitin conjugating enzyme 2, Ubiquitin conjugating enzyme

Alternates names: UbcH2, Ubiquitin conjugating enzyme 2, Ubiquitin conjugating enzyme, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, Ube2h

Taglines: An essential ubiquitin-conjugating enzyme

NCBI Gene ID #: 8743

NCBI Gene Symbol: TNF10

Gene Source: Human

Accession #: P50591

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The activity is determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The ED for the effect is 1-3 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.8 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: Supplied at a concentration of 1.0 mg/ml in 20 mM Tris-HCl, pH 7.5, containing 0.5 mM DTT.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.5-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A