Ubiquitin-Biotinylated (all lysines unmodified) | 7551

Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the -amino group of a lysine (Lys) residue on the target substrate. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains. ’s biotinylated ubiquitin carries a single biotin molecule attached at a defined location and unlike other biotinylated ubiquitins, avoids modification of the N-terminus, C-terminus or any of the seven Lys side chains. Therefore, this biotinylated ubiquitin has all lysines available for conjugation and can be incorporated into polyubiquitin chains of any linkage type.

7551 | Ubiquitin-Biotinylated all lysines unmodified DataSheet

Biomolecule/Target: Ubiquitin

Synonyms: UBB, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A.

Alternates names: UBB, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A.

Taglines: Substrate to determine the activity of ubiquitin conjugating enzymes

NCBI Gene ID #: 22339

NCBI Gene Symbol: VEGFA

Gene Source: N/A

Accession #: Q00731

Recombinant: N/A

Source: N/A

Purity by SDS-PAGEs: 98%

Assay: RP-HPLC

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by the mitogenic activity on human mbilical vein endothelial cells and bovine aortic endothelial cells. Mitogenic activity was obtained for stimulation of 3H-thymidine incorporation and cell proliferation in a concentration range of 1.0 to 5.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 39.0 kDa

Concentration: 8 mg/mL

Appearance: Liquid

Physical form description: N/A

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A