Urokinase | 4793

Urokinase (UK) is a serine protease, which is one of biological plasminogen activators. It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. It can be obtained from human urine or kidney cell culture. UK is a two-chain glycoprotein containing 411 amino acids with 12 dissulfide bonds. Its molecular weight is 54,000.

4793 | Urokinase DataSheet

Biomolecule/Target: N/A

Synonyms: Urokinase, Abbokinase, Urokinase-type Plasminogen Activator,uPA, EC 3.4.21.73, UK.

Alternates names: Urokinase, Abbokinase, Urokinase-type Plasminogen Activator,uPA, EC 3.4.21.73, UK.

Taglines: A serine protease which activates zymogen

NCBI Gene ID #: 3952

NCBI Gene Symbol: LEP

Gene Source: N/A

Accession #: P41159

Recombinant: No

Source: Human urine

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 90%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): 1 nM UK will cause a change in absorbance of 0.001 at 405 nm in 1 minute at R/T in 100 µl reaction mixture containing 0.05 M Tris-HCl, 0.1 M NaCl, pH 7.4, using S2444 (0.6mM) as the substrate.

Biological activity: ’s human Leptin has been shown to be biologically active in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 mg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 16.0 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized powder

Reconstitution Instructions: Add 15 mM sterile HCl (0.5 ml/1 mg vial or 5 ml/10 mg vial) to the vial. After the protein is completely dissolved, add 7.5 mM sterile NaOH (0.3 ml/1 mg vial or 3 ml/10 mg vial) to bring the pH to approximately 5.2.

Amino acid sequence: N/A