USP8 Antibody | 6658

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 (USP8/UBPy) is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indispensible for cell proliferation and survival. Indeed, conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases, including EGFR, ErbB3, and c-Met. In agreement with these findings, USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR. Furthermore, phosphorylation of USP8 at Ser680 results in its binding of 14-3-3, catalytic inactivation, and reduced EGFR deubiquitination. It appears as though USP8, in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation.

6658 | USP8 Antibody DataSheet

Antibody Target: USP8

Target Alternative Name: Deubiquitinating enzyme 8, hUBPy, HumORF8, KIAA0055, mgC129718, Ubiquitin carboxyl-terminal hydrolase 8, Ubiquitin-specific processing protease 8, Ubiquitin thioesterase 8, UBP, Ubiquitin Specific Protease 8.

Tag Line: Polyclonal Antibody to detect USP8 in human samples

Category: USP8

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human

Immunogen Sequence: Synthetic peptide corresponding to an internal sequence of human USP 8 (BV-L43)

Accession #: P40818

Gene ID: 9101

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.5 mg/ml

Formulation: In phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.

Purification: Affinity Purified Antibody

Application: Western blot

Positive Control: Western blot: Jurkat Cell lysate

Application And Usages: Western blot: 1-4 µg/ml.

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified:

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified