763

0.25% Trypsin Solution (with EDTA, phenol red, dissolved in D-Hank's) | EP-CM-L0444

(6 reviews) Write a Review
SKU:
763-EP-CM-L0444-GEN
NULL181.00

Description

0.25% Trypsin Solution (with EDTA, phenol red, dissolved in D-Hank's) | EP-CM-L0444 | Gentaur UK, US & Europe Distribution

Application: Supplements & Reagents

Media Sort: Dissociation reagent

Form: Liquid

Storage: -20°C

Shipping: Ice bag or dry ice

Expiration (month) : 12

Background: Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37°C, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0. 25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.

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Additional Information

Size:
100 mL
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6 Reviews

  • 3
    good support

    Posted by Ashton on 26th Sep 2022

    a friendly relationship between provider and customer is most important and I must say Gentaur staff provides good support to their users

  • 4
    obrigated

    Posted by Brayden on 17th Sep 2022

    gentaur is the only the one provide with obrigated quality. Really appreciated

  • 4
    reasonable products

    Posted by Damian on 17th Sep 2022

    I ordered the product. When I used surprised by its results. Really a great product with a reasonable price

  • 4
    recommendable

    Posted by Beau on 17th Sep 2022

    This is a highly recommendable company, as my experience with Gentaur has been excellent and all the products are high quality.

  • 4
    quick service

    Posted by Ryder on 17th Sep 2022

    Gentaur staff is very friendly. Assist in all the problems very quick. Must recommend this provider due to its quick service

  • 4
    Speedy delivery

    Posted by Micah on 17th Sep 2022

    A highly recommended provider, Gentaur has an administrator who is extremely responsive and has a very speedy delivery. A big thank you to Gentaur.

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