763
0.25% Trypsin Solution (with EDTA, phenol red, dissolved in D-Hank's) | EP-CM-L0444
- SKU:
- 763-EP-CM-L0444-GEN
Description
0.25% Trypsin Solution (with EDTA, phenol red, dissolved in D-Hank's) | EP-CM-L0444 | Gentaur UK, US & Europe Distribution
Application: Supplements & Reagents
Media Sort: Dissociation reagent
Form: Liquid
Storage: -20°C
Shipping: Ice bag or dry ice
Expiration (month) : 12
Background: Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37°C, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0. 25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.
6 Reviews
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good support
a friendly relationship between provider and customer is most important and I must say Gentaur staff provides good support to their users
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obrigated
gentaur is the only the one provide with obrigated quality. Really appreciated
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reasonable products
I ordered the product. When I used surprised by its results. Really a great product with a reasonable price
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recommendable
This is a highly recommendable company, as my experience with Gentaur has been excellent and all the products are high quality.
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quick service
Gentaur staff is very friendly. Assist in all the problems very quick. Must recommend this provider due to its quick service
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Speedy delivery
A highly recommended provider, Gentaur has an administrator who is extremely responsive and has a very speedy delivery. A big thank you to Gentaur.