3-nitroY | Nitrotyrosine | AS10 706-100

3-nitroY | Nitrotyrosine | AS10 706-100 | Gentaur UK, US & Europe Distribution

Immunogen: BSA-conjugated 3- (4-Hydroxy-3-nitrophenyl acetamido) propionic acid

Host: Mouse

Conjugation: N/A

Clonality: Monoclonal

Isotype: IgG2A

Purity: Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.

Format: Liquid

Tested Application: ELISA (ELISA), Flowcyt (Flow cytometry), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)

Related Products: AS10 706-25 | Anti-nitrotyrosine, mouse monoclonal antibodies, 25 µgSecondary antibodies

Recommended Dilutions: 1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator

Molecular weight: N/A

Confirmed Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Higher plants

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivitywith non-nitrated tyrosine. Not species specific.0.7µg/ml was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot..ECL.Antibody works paraffin-embedded sections.

Background: Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.

Reconstitution: N/A

Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Oxidative stress

Research Area: Proteomics