4E-BP1 Antibody [9E12D9] | 32-101

4E-BP1 Antibody [9E12D9] | 32-101 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Ni-NTA purified truncated recombinant 4EBP1 expressed in E. Coli strain BL21 (DE3) .

Research Area: Signal Transduction

Tested Application: E, WB

Application: Western Blot:1:500 - 1:1, 000.
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: 9E12D9

Isotype: IgG1

Conjugate: Unconjugated

Physical State: N/A

Buffer: Ascitic fluid containing 0.03% sodium azide.

Concentration: N/A

Storage Condition: 4E-BP1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Phosphorylated heat- and acid-stable protein regulated by insulin 1, 4E-BP1, BP-1, 4EBP1, PHAS-I

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: 4E-BP1 (eukaryotic translation Initiation Factor 4E Binding Protein 1) , also called ELF4EBP1/BP-1/PHAS-I, which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa) . Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while (24 kDa) , the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.