619 Biotinylation Kit; Reagents for the Biotinylation of 1 – 10 mg Antibody/ other protein

619 Biotinylation Kit; Reagents for the Biotinylation of 1 – 10 mg antibody/ other protein | BTBIOK-05 \BTBIOK-10 | 619

Product Features:

Type: Biotin Labelling Kit

Reactivity : Streptavidin

Antigen : Streptavidin

Detection Range : N/A

Application : Biotin Labelling , e.g. for antibodies, proteins etc.; Biotinylated molecules bind very well to Streptavidin or Streptavidin surfaces

Description : The 619 Biotinylation Kit contains the reagents for the labeling of antibodies and other proteins and the purification of the biotinylated product. The coupling reaction takes place at primary amino groups on the target molecule using a N-Hydroxysuccinimide (NHS) ester-activated biotin, which form stable amide bonds. Proteins generally have a sufficient number of amino groups for coupling, both N-terminal and lysine residues. In this kit, Sulfo-NHS-LC Biotin (sulfosuccinimidyl-6-[biotin-amido] hexanoate) is used, that contains a spacer group with 6 carbon atoms and a length of 22,4 Å to increase the distance between the target molecule and the biotin groups. In the kit are 5 portions of Sulfo-NHS-LC Biotin reagent. One portion is used for a antibody amount of 1-10 mg or a protein amount of 1-5 mg.

Research Area : all immuno assays

Specificity : Streptavidin

Immunogen : N/A

Sensitivity : N/A

Predicted Reactivity : Streptavidin

Cross Reactivity : N/A

Minimum Shelf life time in months : 3 months

Storage Shipping Temp Min : > 0 to +37°C

Storage Shipping Temp Max : 37°C, Biotin reagent at 2-8°C

Storage Long Term : 2-8°C/ -20°C

Principle : Biotinylation with Sulfo-NHS-LC of antibody / protein, coupling reaction with target molecules through the reaction with primary amino groups

Components : Sulfo-NHS-LC Biotin, biotinylation buffer for the coupling reaction, clean-up column and purification buffer concentrate (10x) for size-exclusion chromatography. All components are included for 5 separate coupling reactions.

Characteristics : Kit

Sample Type :

Format : Kit