ACSL1 Antibody | 7081

ACSL1 Antibody | 7081 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Guinea pig: (88%)

Immunogen: Rabbit polyclonal ACSL1 antibody was raised against an 18 amino acid peptide near the center of human ACSL1.
The immunogen is located within amino acids 240 - 290 of ACSL1.

Research Area: Homeostasis

Tested Application: E, WB, IF

Application: ACSL1 antibody can be used for detection of ACSL1 by Western blot at 0.25 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least three isoforms of ACSL1 are known to exist; this antibody will detect all three isoforms.

Positive Control 1: Cat. No. 1304 – Human Liver Tissue Lysate

Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 41, 58, 77 kDa
Observed: 45, 47 kDa 

Validation: N/A

Isoform: N/A

Purification: ACSL1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ACSL1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ACSL1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: ACSL1 Antibody: ACS1, LACS, FACL1, FACL2, LACS1, LACS2, Long-chain-fatty-acid--CoA ligase 1, Acyl-CoA synthetase 1, ACS1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ACSL1 Antibody: Long-chain acyl coenzyme A synthetase 1 (ACSL1) catalyzes the synthesis of acyl-CoA from long-chain fatty acids in an ATP-dependent manner. ACSL1 is a member of a family of long-chain acyl-CoA synthetases which differ in substrate preference, tissue expression, and subcellular localization. In mouse, ACSL1 is the major acyl-CoA enzyme in the heart, providing 60-90% of heart ATP. Loss of ACSL1 either globally or in heart ventricles resulted in impaired fatty acid oxidation, activation of the mammalian target of rapamycin (mTOR) , and cardiac hypertrophy.