ACTN4 Antibody | 19-019

ACTN4 Antibody | 19-019 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: A synthetic peptide of human ACTN4

Research Area: Cancer, Cell Cycle, Immunology, Signal Transduction

Tested Application: WB, IHC, Flow

Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Flow: 1:20 - 1:50

Specificiy: N/A

Positive Control 1: HeLa

Positive Control 2: HepG2

Positive Control 3: HT-1080

Positive Control 4: MCF7

Positive Control 5: NIH/3T3

Positive Control 6: N/A

Molecular Weight: Observed: 105kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, pH7.3.

Concentration: N/A

Storage Condition: Store at 4˚C. Avoid freeze / thaw cycles.

Alternate Name: ACTN4, DKFZp686K23158, FSGS, FSGS1, ACTININ-4

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.