Adiponectin Antibody [ADPQ-1] | 34-067

Adiponectin Antibody [ADPQ-1] | 34-067 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: A human partial recombinant protein was used as the immunogen for this Adiponectin antibody.

Research Area: Obesity, Signal Transduction, Neuroscience

Tested Application: E, Flow, IF, IHC

Application: Flow Cytometry: 0.5-1ug/10^6 cells
IF: 1-2 ug/ml
ELISA: order BSA free format for coating
IHC (FFPE) : 1-2 ug/ml (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Adiponectin antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: ADPQ-1

Isotype: IgG2b

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Adiponectin Antibody: ACDC, ADPN, APM1, APM-1, GBP28, ACRP30, ADIPQTL1, ACDC, Adiponectin, 30 kDa adipocyte complement-related protein

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Adiponectin (commonly called ADIPO, or by the gene name ADIPOQ) is an adipocytokine, a hormone produced in adipose tissue. It is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma ADIPO levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women, levels tend to be higher than in men, which may be due to androgens suppressing ADIPO levels. Furthermore, adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low ADIPO levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism.