ADORA2B Antibody | 8111

ADORA2B Antibody | 8111 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Rabbit: (80%)

Immunogen: ADORA2B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADORA2B.
The immunogen is located within the last 50 amino acids of ADORA2B.

Research Area: Immunology, Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: ADORA2B antibody can be used for detection of ADORA2B by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: ADORA2B antibody is human, mouse and rat reactive. At least two isoforms of ADORA2B are known to exist; this antibody will detect both isoforms. ADORA2B antibody is predicted to not cross-react with ADORA2A.

Positive Control 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate

Positive Control 2: Cat. No. 1411 - Mouse Colon Tissue Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 37 kDa
Observed: 41 kDa

Validation: N/A

Isoform: N/A

Purification: ADORA2B antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ADORA2B antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ADORA2B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Adenosine receptor A2b, ADORA2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1, 2) . Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5) . Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs) , a class of cells that work to constrain inflammation (6) .