ADROPIN Antibody | 7527

ADROPIN Antibody | 7527 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)

Immunogen: ADROPIN antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ADROPIN.
The immunogen is located within the last 50 amino acids of ADROPIN.

Research Area: Obesity

Tested Application: E, WB, IHC-P, IF

Application: ADROPIN antibody can be used for detection of ADROPIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: ADROPIN antibody is human, mouse and rat reactive. Despite its predicted molecular weight, ADROPIN often migrates at a higher than expected molecular weight in SDS-PAGE.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 8 kDa
Observed: 16 kDa

Validation: N/A

Isoform: N/A

Purification: ADROPIN antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ADROPIN antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ADROPIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: ADROPIN Antibody: UNQ470, C9orf165, UNQ470/PRO830, Adropin, Energy homeostasis-associated protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ADROPIN is a recently identified protein that has been implicated in the maintenance of energy homeostasis and insulin resistance (1-3) . ADROPIN expression is regulated by energy status and dietary nutrient content and is altered by obesity and regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor gamma (PPAR-gamma) (1) . ADROPIN levels increase with dietary fat content (2) . ADROPIN has also been proposed to play a role in the regulation of endothelial function (3) .