AFAP1 Antibody | 6557

AFAP1 Antibody | 6557 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (78%)

Immunogen: AFAP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AFAP1.
The immunogen is located within amino acids 30 - 80 of AFAP1.

Research Area: Cancer, Cell Cycle

Tested Application: E, WB

Application: AFAP1 antibody can be used for detection of AFAP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Specificiy: Monomer and homomultimer of AFAP1 are known to exist; AFAP1 antibody is predicted to not cross-react with other AFAP family members.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: AFAP1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: AFAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: AFAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: AFAP1 Antibody: AFAP, AFAP110, AFAP-110, AFAP, Actin filament-associated protein 1, 110 kDa actin filament-associated protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: AFAP1 Antibody: The actin filament-associated protein AFAP1 (AFAP-110) is an actin cross-linking protein first identified as a substrate of the viral oncogene v-Src. AFAP1 has a fundamental role in actin cytoskeleton arrangement. It contains a carboxyterminal actin-binding domain that directly binds to F-actin and serves as an adaptor protein in the regulation of SRC and PKC signal transduction by several functional domains, including 2 pleckstrin homology (PH) domains, a Src homology 3-binding (SH3-binding) motif, and several SH2-binding motifs. It is overexpressed in prostate carcinoma and contributes to tumor growth by regulating cell-matrix adhesions and migration in the cancer cells. AFAP1 represent a possible therapeutic target for controlling tumorigenesis and metastasis.