AIF Antibody | 2239

AIF Antibody | 2239 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (50%) , Rat: (50%)

Immunogen: Anti-AIF antibody (2239) was raised against a peptide corresponding to 14 amino acids near the amino terminus of mature human AIF.
The immunogen is located within amino acids 90-140 of AIF.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, IF

Application: WB: 1-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Multiple isoforms of AIF are known to exist.

Positive Control 1: Cat. No. 1204 - K562 Cell Lysate

Positive Control 2: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3: Cat. No. 17-005 - Jurkat Cell Slide

Positive Control 4: Cat. No. 17-004 - K-562 Cell Slide

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 67 kDa
Observed: 68 kDa

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar AIF expression profile in human and mouse cell lines detected by three independent anti-AIF antibodies that recognize different epitopes, 2267 against central domain, 2239 against N-terminus domain and 2301 against C-terminus domain.  AIF proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies. 

KD Validation (Figure 4, 5) : Anti-AIF antibody specificity was further verified by AIF specific knockdown. AIF signal in H1299 cells and AIF stable cells transfected with AIF siRNAs was disrupted in comparison with that in cells transfected with control siRNAs.

Localization Validation (Figure 7) : AIF detected by anti-AIF antibodies is colocalized with Mitotracker (mitochondrion marker) .  AIF is localized in mitochondria.

Induced Expression Validation (Figure 8) : AIF expression detected by anit-AIF antibodies was up-regulated by Magnolol treatment.

Isoform: Human AIF has 6 isoforms, including isoform 1 (613aa, 67kD) , isoform 2 (326aa, 36kD) , isoform 3 (609aa, 66kD) , isoform 4 (324aa, 35kD) , isoform 5 (261aa, 28kD) , and isoform 6 (237aa, 26kD) . Mouse AIF has one isoform (612aa, 67kD) and Rat AIF also has one isoform (612aa, 67kD) . 2239 can detect only 4 human isoforms (1, 3, 4, 6) .

Purification: AIF Antibody is Ion exchange chromatography purified.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: AIF Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: AIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: AIF Antibody: Apoptosis-inducing factor 1, Programmed cell death protein 8, AIF, PDCD8

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) . Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed.