AKAP4 Antibody | 7961

AKAP4 Antibody | 7961 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Rat: (94%) , Mouse: (88%)

Immunogen: AKAP4 antibody was raised against a 17 amino acid peptide near the amino terminus of human AKAP4.
The immunogen is located within the first 50 amino acids of AKAP4.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: AKAP4 antibody can be used for detection of AKAP4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms.

Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate

Positive Control 2: Cat. No. 11-701 - Human Testis Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 94 kDa
Observed: 100 kDa

Validation: N/A

Isoform: N/A

Purification: AKAP4 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: AKAP4 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: AKAP4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: A-kinase anchor protein 4, AKAP-4, AKAP82, hAKAP82, CT99, FSC1, HI, PRKA4

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell (1) . AKAP4, also known as hAKAP82, is a major sperm fibrous sheath protein that localizes to the entire length of the flagellum of human sperm (2) . It has been suggested that AKAP4 plays multiple roles in sperm motility and regulation of signal transduction pathways (2) . AKAP4 has also been postulated to be a potential biomarker for breast cancer (3) .