AKT1S1 Antibody | 7391

AKT1S1 Antibody | 7391 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: AKT1S1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1.
The immunogen is located within amino acids 190 - 240 of AKT1S1.

Research Area: Autophagy

Tested Application: E, WB, IHC-P, IF

Application: AKT1S1 antibody can be used for detection of AKT1S1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: AKT1S1 antibody is human, mouse and rat reactive. At least three isoforms of AKT1S1 are known to exist.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 30 kDa
Observed: 28 kDa

Validation: N/A

Isoform: N/A

Purification: AKT1S1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: AKT1S1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: AKT1S1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: AKT1S1 Antibody: Lobe, PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: AKT1S1 Antibody: The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia (1) . AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR) , a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy (2) . Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells (3) .