ALCAM/CD166 (Human) ELISA Kit - E5029

E5029 | ALCAM/CD166 (Human) ELISA Kit DataSheet

ALCAM/CD166 antigen is a 100-105 kDa type I transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins, which is also known as activated leukocyte cell adhesion molecule (ALCAM) in human, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. CD166 is a cell adhesion molecule that binds to CD6. CD166 is involved in neurite extension by neurons via heterophilic and homophilic interactions. It is increased in various types of cancer. ’s ALCAM/CD166 (Human) ELISA Kit is used for the quantitative detection of ALCAM in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ALCAM in the sample.

Alternate Name : Human ALCAM/CD166 ELISA Kit; ALCAM ELISA kit; CD166 ELISA kit; MEMD ELISA kit

Tag Line : A Sandwich ELISA kit for quantitative measurement of ALCAM

Summary :

Detection Method : Absorbance is measured at 450 nm

Sample Type : Human Serum, Plasma, Tissue lysates and other biological fluids

Species Reactivity : Human

Applications : The ELISA kit is used for the quantitative detection of ALCAM in serum, plasma, tissue homogenates and other biological fluids

Features and Benefits : Sensitivity: 37.5 pg/ml Highly sensitive and specific Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Detection range: 62.5-4000 pg/ml Recovery Rate: 90-103% for serum, 87-103% for EDTA plasma and 88-100% for heparin plasma