Alkaline Phosphatase, Calf Intestine | 7585

Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is pH 10 in standard conditions. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.

7585 | Alkaline Phosphatase Calf Intestine DataSheet

Biomolecule/Target: Alkaline Phosphatase

Synonyms: ALP-1, ALPPL2, ALPPL, ALPP, ALP, PALP, PLAP, PLAP-1.

Alternates names: ALP-1, ALPPL2, ALPPL, ALPP, ALP, PALP, PLAP, PLAP-1

Taglines: A hydrolase enzyme responsible for removing phosphate groups

NCBI Gene ID #: 10673

NCBI Gene Symbol: TN13B

Gene Source: Calf

Accession #: Q9Y275

Recombinant: N/A

Source: Calf Intestine

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED for this effect is less than 10 ng/ml. Please note: results may vary with PBMC donors.

Results: 3,000-6,000 U/mg protein

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.335 kDa

Concentration: N/A

Appearance: Freeze Dried

Physical form description: Lyophilized without any additives.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A