ALKBH5 Antibody (Center) [APR31675G]

ALKBH5 Antibody (Center) [APR31675G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This ALKBH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the Central region of human ALKBH5.

Cellular Localisation: Nucleus speckle

Molecular Weight: 44256

Clone: Polyclonal

Gene Name: ALKBH5

Gene ID: 54890

Function: Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N (6) -methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178) . Can also demethylate N (6) -methyladenosine in single- stranded DNA (in vitro) (PubMed:24616105) . Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178) . Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736) . Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity) .

Summary: Tissue Location: Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, FC

Dilution: IHC-P--1:100 FC--1:25 WB--1:500

Synonyms: RNA demethylase ALKBH5, 11411-, Alkylated DNA repair protein alkB homolog 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, ALKBH5, ABH5, OFOXD1