ALKBH8 Antibody (C-term) [APR05806G]

ALKBH8 Antibody (C-term) [APR05806G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This ALKBH8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from the C-terminal region of human ALKBH8.

Cellular Localisation: Cytoplasm. Nucleus Note=Predominantly cytoplasmic.

Molecular Weight: 75208

Clone: Polyclonal

Gene Name: ALKBH8

Gene ID: 91801

Function: Catalyzes the methylation of 5-carboxymethyl uridine to 5- methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323, PubMed:31079898) . Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323) . Has a preference for tRNA (Arg) and tRNA (Glu), and does not bind tRNA (Lys) (PubMed:20308323) . Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5- (S) - methoxycarbonylhydroxymethyluridine; has a preference for tRNA (Gly) (PubMed:21285950) . Required for normal survival after DNA damage (PubMed:20308323) . May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182) .

Summary: Tissue Location: Widely expressed, with highest expression in spleen, followed by pancreas and lung.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, FC, WB

Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50

Synonyms: Alkylated DNA repair protein alkB homolog 8, 11411-, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8, tRNA (carboxymethyluridine (34) -5-O) -methyltransferase ABH8, ALKBH8, ABH8