ALMS1 Antibody | 45-240

ALMS1 Antibody | 45-240 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow

Immunogen: The immunogen for this antibody is: C-RVTNQLLGRKVPWD

Research Area: Cancer

Tested Application: E, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 200kDa and 75kDa in lysates of Human T cell line Jurkat after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461kDa according to NP_055935.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .Immunohistochemistry:In paraffin embedded Human Testis shows staining of cytoplasm in spermatocytes. Recommended concentration: 1-3ug/ml.

Specificiy: The C terminus of ALMS1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693) . However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis.

Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: ALMS1, Alstrom syndrome 1, ALSS, KIAA0328, DKFZp686A118, DKFZp686D1828

User Note: N/A

BACKGROUND: N/A