ALOX15 Antibody (C-term) [APG01823G]

ALOX15 Antibody (C-term) [APG01823G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This ALOX15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-643 amino acids from the C-terminal region of human ALOX15.

Cellular Localisation: Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Lipid droplet. Note=Predominantly cytosolic; becomes enriched at membranes upon calcium binding (By similarity) Translocates from the cytosol to the plasma membrane when stimulated by IL13/interleukin-13 and in macrophages binding apoptotic cells (By similarity) . {ECO:0000250|UniProtKB:P39654}

Molecular Weight: 74804

Clone: Polyclonal

Gene Name: ALOX15 (HGNC:433)

Gene ID: 246

Function: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators (PubMed:1944593, PubMed:8334154, PubMed:17052953, PubMed:24282679, PubMed:25293588, PubMed:32404334) . It inserts peroxyl groups at C12 or C15 of arachidonate ( (5Z, 8Z, 11Z, 14Z) -eicosatetraenoate) producing both 12-hydroperoxyeicosatetraenoate/12-HPETE and 15- hydroperoxyeicosatetraenoate/15-HPETE (PubMed:1944593, PubMed:8334154, PubMed:17052953, PubMed:24282679) . It may then act on 12-HPETE to produce hepoxilins, which may show proinflammatory properties (By similarity) . Can also peroxidize linoleate ( (9Z, 12Z) -octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (PubMed:8334154) . May participate in the sequential oxidations of DHA ( (4Z, 7Z, 10Z, 13Z, 16Z, 19Z) -docosahexaenoate) to generate specialized pro- resolving mediators (SPMs) like resolvin D5 ( (7S, 17S) -diHPDHA) and (7S, 14S) -diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334) . Can convert epoxy fatty acids to hydroperoxy-epoxides derivatives followed by an intramolecular nucleophilic substitution leading to the formation of monocyclic endoperoxides (PubMed:25293588) . Plays an important role during the maintenance of self-tolerance by peroxidizing membrane-bound phosphatidylethanolamine which can then signal the sorting process for clearance of apoptotic cells during inflammation and prevent an autoimmune response. In addition to its role in the immune and inflammatory responses, this enzyme may play a role in epithelial wound healing in the cornea through production of lipoxin A4 (LXA (4) ) and docosahexaenoic acid-derived neuroprotectin D1 (NPD1; 10R, 17S-HDHA), both lipid autacoids exhibit anti-inflammatory and neuroprotective properties. Furthermore, it may regulate actin polymerization which is crucial for several biological processes such as the phagocytosis of apoptotic cells. It is also implicated in the generation of endogenous ligands for peroxisome proliferator activated receptor (PPAR-gamma), hence modulating macrophage development and function. It may also exert a negative effect on skeletal development by regulating bone mass through this pathway. As well as participates in ER stress and downstream inflammation in adipocytes, pancreatic islets, and liver (By similarity) . Finally, it is also involved in the cellular response to IL13/interleukin-13 (PubMed:21831839) .

Summary: Tissue Location: Detected in monocytes and eosinophils (at protein level) . Expressed in airway epithelial cells

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, IF

Dilution: WB--1:2000

Synonyms: Arachidonate 15-lipoxygenase, 15-LOX, 15-LOX-1, 12/15-lipoxygenase, Arachidonate 12-lipoxygenase, leukocyte-type, 12-LOX, Arachidonate omega-6 lipoxygenase, ALOX15, LOG15