ALOX15B Antibody (C-term) [APG01825G]

ALOX15B Antibody (C-term) [APG01825G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This ALOX15B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 597-627 amino acids from the C-terminal region of human ALOX15B.

Cellular Localisation: [Isoform A]: Nucleus. Note=Other isoforms are excluded from the nucleus.

Molecular Weight: 75857

Clone: Polyclonal

Gene Name: ALOX15B (HGNC:434)

Gene ID: 247

Function: [Isoform A]: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (PubMed:9177185, PubMed:10625675, PubMed:12704195, PubMed:17493578, PubMed:18311922, PubMed:24282679, PubMed:10542053, PubMed:24497644, PubMed:32404334) (Probable) . It inserts peroxyl groups at C15 of arachidonate ( (5Z, 8Z, 11Z, 14Z) -eicosatetraenoate) producing (15S) -hydroperoxyeicosatetraenoate/ (15S) -HPETE (PubMed:17493578, PubMed:12704195, PubMed:24282679, PubMed:9177185, PubMed:11956198, PubMed:10625675, PubMed:24497644) (Probable) . Also peroxidizes linoleate ( (9Z, 12Z) -octadecadienoate) to 13- hydroperoxyoctadecadienoate/13-HPODE (Probable) (PubMed:10542053, PubMed:27435673) . Oxygenates arachidonyl derivatives such as 2- arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist (PubMed:18311922, PubMed:17493578, PubMed:11956198) . Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti- inflammatory intermediates (PubMed:27145229) . Participates in the sequential oxidations of DHA ( (4Z, 7Z, 10Z, 13Z, 16Z, 19Z) -docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ( (7S, 17S) -diHPDHA), which can actively downregulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334) . In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids (PubMed:27435673) . Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S) -HPETE to produce (8S, 15S) -diHPETE (Probable) . May regulate progression through the cell cycle and cell proliferation (PubMed:12704195, PubMed:11839751) . May also regulate cytokine secretion by macrophages and therefore play a role in the immune response (PubMed:18067895) . May also regulate macrophage differentiation into proatherogenic foam cells (PubMed:22912809) .

Summary: Tissue Location: Expressed in hair, prostate, lung, ovary, lymph node, spinal cord and cornea.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Arachidonate 15-lipoxygenase B, 15-LOX-B, 15-lipoxygenase 2, 15-LOX-2, Arachidonate 15-lipoxygenase type II, Linoleate 13-lipoxygenase 15-LOb, 11311-, ALOX15B