ALOXE3 Antibody (Center) [APR30609G]

ALOXE3 Antibody (Center) [APR30609G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This ALOXE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-252 amino acids from the Central region of human ALOXE3.

Cellular Localisation: Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU00726}.

Molecular Weight: 80543

Clone: Polyclonal

Gene Name: ALOXE3 (HGNC:13743)

Gene ID: 59344

Function: Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced lipoxygenases activity (PubMed:12881489, PubMed:17045234, PubMed:20921226, PubMed:20923767) . The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols and ketones (PubMed:12881489, PubMed:17045234, PubMed:20923767) . In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides (PubMed:20921226) . In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins (PubMed:21558561) . Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed:21558561) . In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites (PubMed:21558561) . Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG (By similarity) . Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia (By similarity) .

Summary: Tissue Location: Predominantly expressed in skin.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P-Leica, WB

Dilution: IHC-P-Leica--1:500 WB--1:1000

Synonyms: Hydroperoxide isomerase ALOXE3, Epidermis-type lipoxygenase 3, Epidermal LOX-3, e-LOX-3, eLOX-3, Hydroperoxy icosatetraenoate dehydratase, ALOXE3